Restriction Enzymes
New England Biolabs, Inc. Bsu36I – 1000 units
A restriction endonuclease that recognizes the sequence CC^TNA_GG.
New England Biolabs, Inc. BlpI – 500 units
A restriction endonuclease that recognizes the sequence GC^TNA_GC.
New England Biolabs, Inc. PvuII – 5000 units
A restriction endonuclease that recognizes the sequence CAG_^CTG.
New England Biolabs, Inc. SphI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. AatII – 500 units
A restriction endonuclease that recognizes the sequence G_ACGT^C.
New England Biolabs, Inc. DraIII-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence CAC_NNN^GTG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. MboI – 500 units
A restriction endonuclease that recognizes the sequence ^GATC_.