Restriction Enzymes
New England Biolabs, Inc. EnGen Spy Cas9 Nickase – 70 pmol
EnGen Spy Cas9 Nickase is a variant of Cas9 nuclease differing by a point mutation (D10A) in the RuvC nuclease domain, which enables it to nick, but not cleave, DNA). EnGen Spy Cas9 Nickase, like EnGen Cas9 NLS , targets DNA using a guide RNA complementary to a site with a 3' NGG protospacer adjacent motif (PAM). Nicking occurs on the DNA strand opposite the PAM. Double-stranded DNA breaks can be generated with reduced off-target cleavage by targeting two sites in close proximity (generally 0-20 bp apart) and with PAMs facing outward to leave 5' overhangs. Nicking by EnGen Spy Cas9 Nickase occurs on the DNA strand opposite the target sequence. EnGen Spy Cas9 Nickase contains SV40 T antigen nuclear localization (NLS) sequences on the N and C- termini.
New England Biolabs, Inc. HpyAV – 100 units
A restriction endonuclease that recognizes the sequence CCTTCNNNNN_N^.
New England Biolabs, Inc. EcoP15I – 500 units
A restriction endonuclease that recognizes the sequence CAGCAGNNNNNNNNNNNNNNNNNNNNNNNNN^NN_.
Sigma Aldrich Fine Chemicals Biosciences β-Glucuronidase from Escherichia coli | CAS 9027-70-9 | 1,000,000 Units
β-glucuronidase from E. coli | Purity: ≥90% | Mol Wt: 68 kDa | CAS No: 9027-70-9 | Unit: 1,000,000 Units
New England Biolabs, Inc. Eco53kI – 1000 units
A restriction endonuclease that recognizes the sequence GAG_^CTC.
New England Biolabs, Inc. BcoDI – 1000 units
A restriction endonuclease that recognizes the sequence GTCTCN^NNNN_.
New England Biolabs, Inc. MspJI – 200 units
A restriction endonuclease that recognizes the sequence CNNRNNNNNNNNN^NNNN_.
New England Biolabs, Inc. MluCI – 1000 units
A restriction endonuclease that recognizes the sequence ^AATT_.
New England Biolabs, Inc. MluCI – 5000 units
A restriction endonuclease that recognizes the sequence ^AATT_.
New England Biolabs, Inc. EagI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence C^GGCC_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. SphI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. WarmStart Nt.BstNBI-1000 u
WarmStart Nt.BstNBI is a site specific nicking endonuclease cloned fromBacillus Stereothermophilus. Restriction Enzyme Cut Site GAGTC(4/-5). It is formulated with a reversibly-bound aptamer which inhibits its nicking activity at temperatures below 40C. WarmStart Nt.BstNBI cleaves only one strand of DNA on a double-stranded DNA substrate. The nicking endonuclease catalyzes a single strand break 4 bases beyond the 3side of the recognition sequence.